The SPRINT4.0 Handbook describes an innovative approach that mixes theory and practice into a program where Universities, Technology Providers and Target Companies work together to create new professional profiles able to manage the impact of the fourth industrial revolution on companies. This guide will help European Universities and Research Institutes to design new courses and to adapt their curricula to the innovations and the opportunities brought by Industry 4.0. The guide is designed to be easily transferable and exploitable from any stakeholder interested in sharing Industry 4.0 skills and expertise.